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nCounter® Myeloid Innate Immunity Panel

The nCounter Myeloid Innate Immunity panels are designed to encompass all aspects of the myeloid innate immune response for use in basic and translational research studies. The panel provides an effective means to comprehensively measure quantitatively and qualitatively the attributes of the innate immune response by evaluating relative enrichment of genes that mark recruitment and presence of selected myeloid subtype.

Product Highlights

  • Useful for research areas including Immuno-Oncology, Autoimmune Disease and Infectious Disease research.
  • Genes representing all major myeloid cell types and their various functions including: neutrophils, eosinophils, mast cells, dendritic cells, monocytes, and macrophages with 19 functional and pathway annotations.
  • Customize with Panel-Plus, a custom spike-in product of 30-genes or less that allows for the creation of a panel and experiment uniquely your own!

Product Information

  • Product Specs
  • Support Documents
  • Publications
  • Product Details

Product Specifications

Feature Specifications
Number of Targets 770 (Human), 754 (Mouse) including internal reference genes
Standard Input Material 25 ng–300 ng
Low Input Material As little as 1ng with nCounter RNA Low Input Kit and Panel specific primer pools (sold seperately)
Sample Type(s) FFPE-derived RNA, total RNA, fragmented RNA, cell lysates and sorted cells.
Species Human or Mouse
Low Input Material Protocol Yes
Customizable Yes
Time to Results Approximately 24 hours
Data Analysis nSolver™ Analysis software
Advanced Data Analysiss Data QC and normalization, differential gene expression
Shipping and Storage Conditions -80°C

To receive a quote, please fill out the Request Information form.

Publications

Link: Immunology publications

Monocytes/Macrophages
1. Murray PJ et al. Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines. Immunity, Volume 41, Issue 1, 17 July 2014
2. Xue, J. et al. Transcriptome-based network analysis reveals a spectrum model of human macrophage activation. Immunity 40, 274–288 (2014).
Eosinophils
3. Davis BP and Rothenberg ME. Eosinophils and cancer. Cancer Immunol Res. 2014;2(1):1-8.
Neutrophils
4. Lakschevitz FS, Visser MB, Sun C and Glogauer M. Neutrophil transcriptional profile changes during transit from bone marrow to sites of inflammation. Cell Mol Immunol. 2015;12(1):53-65.
5. Fridlender ZG, Sun J, Mishalian I, Singhal S, Cheng G, Kapoor V, Horng W, Fridlender G, Bayuh R, Worthen GS and Albelda SM. Transcriptomic analysis comparing tumor-associated neutrophils with granulocytic myeloid-derived suppressor cells and normal neutrophils. PLoS One. 2012;7(2):e31524.
6. Gregory AD and Houghton AM. Tumor-associated neutrophils: new targets for cancer therapy. Cancer Res. 2011;71(7):2411-6.
Dendritic Cells
7. Chaussabel D, et al. Unique gene expression profiles of human macrophages and dendritic cells to phylogenetically distinct parasites. Blood. 2003 Jul 15;102(2):672-81.
8. Ruffell B, et al. Macrophage IL-10 Blocks CD8+ T Cell-Dependent Responses to Chemotherapy by Suppressing IL-12 Expression in Intratumoral Dendritic Cells. Cancer Cell, Volume 26, Issue 5, 10 November 2014
9. Broz ML, Binnewies M, Boldajipour B, Nelson AE, Pollack JL, Erle DJ, Barczak A, Rosenblum MD, Daud A, Barber DL, Amigorena S, Van’t Veer LJ,Sperling AI, Wolf DM, Krummel MF. Dissecting the tumor myeloid compartment reveals rare activating antigen-presenting cells critical for T cellimmunity. Cancer Cell. 2014 Nov 10;26(5):638-52.
Mast Cells
10. Kuramasu A, Kubota Y, Matsumoto K, Nakajima T, Sun XM, Watanabe T, Saito H, Ohtsu H. Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells. FEBS Lett. 2001 Jun 1;498(1):37-41.
11. Jayapal M, Tay HK, Reghunathan R, Zhi L, Chow KK, Rauff M, Melendez AJ. Genomewide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRIaggregation reveals a complex network of genes involved in inflammatory responses. BMC Genomics. 2006 Aug 16;7:210.

Product Details

Product Description Catalog Number Unit Size
nCounter Human Myeloid Innate Immunity Panel V2 Code Set Only Includes 770 genes, including 40 internal reference genes for data normalization. XT-CSO-HMII2-12 12 Reactions
nCounter Mouse Myeloid Innate Immunity Panel V2 Includes 754 genes, including 20 internal reference genes for data normalization. XT-CSO-MMII2-12 12 Reactions
nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems. NAA-AKTI-012 12 Reactions
nCounter SPRINT Cartridge 1 Cartridge, 12 lanes Sample Cartridge for nCounter SPRINT System SPRINT-CAR-1.0 12 Reactions
nCounter SPRINT Reagent Pack nCounter SPRINT Reagent Pack containing Reagents A,B,C & Hybridization Buffer SPRINT-REAG-KIT 192 Reactions
Low Input RNA Reagent Kit 48rxn kit for profiling from low sample input amounts LOW-RNA-48 48 Reactions
Human Myeloid Innate Immunity Primer Pools MTE primer pools for Low Input RNA profiling (770 genes) 730 immune related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required PP-HMII2-12 12 Reactions
Mouse Myeloid Innate Immunity MTE primer pools for Low Input RNA profiling (754 genes) 734 immune-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required PP-MMII2-12 12 Reactions

To receive a quote, please fill out the Request Information form.


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