nCounter miRGE™ CodeSets
Analyze Both miRNAs and mRNAs Simultaneously
The nCounter miRGE™ Assay is uniquely designed to provide a single-tube, sensitive, reproducible and highly multiplexed method for detecting subsets of both mRNAs and miRNAs simultaneously across all biological levels of expression.
- Highly multiplexed, simultaneous measurement of subsets of miRNAs and
mRNAs in a single tube reaction
- No reverse transcription, no amplification, and fewer pipetting steps than other assays
means less chance for errors and bias in results
- Truly digital data acquisition delivers sensitive and highly reproducible results
- Compatible with total purified RNA and FFPE-derived total RNA
Two Assays... One Reaction
nCounter miRGE CodeSets are designed to provide a single-tube, sensitive, reproducible and highly multiplexed method for detecting subsets of both mRNAs and miRNAs across all biological levels of expression. After processing with the nCounter miRNA Sample Preparation Kit, messenger RNA and tagged miRNAs in total RNA are quantified without the use of reverse transcription or amplification. Instead, the individual sequences are counted with sequence-specific fluorescent barcodes. The assay can be run on total RNA isolated from a wide variety of sample types, including Formalin-Fixed Paraffin Embedded (FFPE) samples.
The nCounter miRGE Assay delivers accurate and sensitive profiling of 100 to 200 mRNA targets and between 5 and 30 miRNA targets. The system provides exceptional ease-of-use for the combined analysis of miRNA and mRNA expression.
nCounter miRGE™ Specifications
- Ordering Info
|Level of multiplexing||100 - 200 mRNAs
5 - 30 miRNAs
|Recommended amount of starting material||100ng of purified total RNA|
|Sample types supported||Purified total RNA|
|Sample prep reaction volume||10 μL|
|Hybridization reaction volume||35 μL|
|Limit of detection: miRNA||> 2.5 copies per cell copy per cell|
|Limit of detection: mRNA||1 copy per cell|
|Fold change sensitivity||> 2-fold change|
|Hybridization spike correlation||R² ≥ 0.95|
|Linear dynamic range||2 x 10⁶ total counts|
|nCounter Prep Station throughput||12 samples < 2.5 hours|
|nCounter Digital Analyzer throughput||12 samples / 4 hours (up to 72 samples per day unattended running in continuous mode)|
|Controls||6 positive hybridization controls
8 negative hybridization controls
2 positive ligation controls
nCounter miRGE CodeSets are available in 48 assays, 96 assays, 192 assays and then increasing increments of 192 assays. To receive a quote, please fill out the Request Information form.
|Description||Quantity / Use||Part Number (P/N)|
|nCounter miRGE Assay||48 assays
|nCounter Analysis System
(includes the Prep Station and Digital Analyzer)
|Additional nCounter Prep Station||1||NCT-PREP-120|
|Additional nCounter Digital Analyzer||1||NCT-DIGA-120|
To receive a quote, please fill out the Request Information form.