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nCounter miRGE™ CodeSets

Analyze Both miRNAs and mRNAs Simultaneously

The nCounter miRGE™ Assay is uniquely designed to provide a single-tube, sensitive, reproducible and highly multiplexed method for detecting subsets of both mRNAs and miRNAs simultaneously across all biological levels of expression.

Product Highlights

  • Highly multiplexed, simultaneous measurement of subsets of miRNAs and
    mRNAs in a single tube reaction
  • No reverse transcription, no amplification, and fewer pipetting steps than other assays
    means less chance for errors and bias in results
  • Truly digital data acquisition delivers sensitive and highly reproducible results
  • Compatible with total purified RNA and FFPE-derived total RNA

Two Assays... One Reaction

nCounter miRGE CodeSets are designed to provide a single-tube, sensitive, reproducible and highly multiplexed method for detecting subsets of both mRNAs and miRNAs across all biological levels of expression. After processing with the nCounter miRNA Sample Preparation Kit, messenger RNA and tagged miRNAs in total RNA are quantified without the use of reverse transcription or amplification. Instead, the individual sequences are counted with sequence-specific fluorescent barcodes. The assay can be run on total RNA isolated from a wide variety of sample types, including Formalin-Fixed Paraffin Embedded (FFPE) samples.

The nCounter miRGE Assay delivers accurate and sensitive profiling of 100 to 200 mRNA targets and between 5 and 30 miRNA targets. The system provides exceptional ease-of-use for the combined analysis of miRNA and mRNA expression.

nCounter miRGE™ Specifications

  • Specifications
  • Ordering Info

Specifications

Description Specifications
Level of multiplexing 100 - 200 mRNAs
5 - 30 miRNAs
Recommended amount of starting material 100ng of purified total RNA
Sample types supported Purified total RNA
Sample prep reaction volume 10 μL
Hybridization reaction volume 35 μL
Limit of detection: miRNA > 2.5 copies per cell copy per cell
Limit of detection: mRNA 1 copy per cell
Fold change sensitivity > 2-fold change
Hybridization spike correlation R² ≥ 0.95
Linear dynamic range 2 x 10⁶ total counts
nCounter Prep Station throughput 12 samples < 2.5 hours
nCounter Digital Analyzer throughput 12 samples / 4 hours (up to 72 samples per day unattended running in continuous mode)
Controls 6 positive hybridization controls
8 negative hybridization controls
2 positive ligation controls

nCounter miRGE CodeSets are available in 48 assays, 96 assays, 192 assays and then increasing increments of 192 assays. To receive a quote, please fill out the Request Information form.

Ordering Information

Description Quantity / Use Part Number (P/N)
nCounter miRGE Assay 48 assays
96 assays
MIR-P1CC-48
MIR-P1CC-96
nCounter Analysis System
(includes the Prep Station and Digital Analyzer)
1 NCT-SYS-120
Additional nCounter Prep Station 1 NCT-PREP-120
Additional nCounter Digital Analyzer 1 NCT-DIGA-120

To receive a quote, please fill out the Request Information form.


Request Information | E-mail us: info@nanostring.com | Call us: 1 888 358 6266