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nCounter® Low RNA Input Kit

The nCounter Low RNA Input Kit enables the generation of high quality gene expression profiling of up to 800 gene targets from as little as 1 ng of sample. The kit is optimized for use with RNA from Formalin Fixed Paraffin Embedded (FFPE) tissue as well as crude cell lysates. Additionally, the kit can be utilized in the study of low expressing genes. The streamlined, user friendly workflow and reliable results enable gene expression studies of small samples or low expressing genes to be completed quickly and efficiently.

Product Highlights

  • High Sensitivity - Perform gene expression analysis on up to 800-genes from as little as 1 ng of RNA
  • Optimized for FFPE - Analyze FFPE & other degraded samples from as little as 10 ng of RNA
  • Simple Workflow - Only 30 minutes of hands-on time
  • Detect Low Expressing Genes - The Low RNA Input kit increases nCounter sensitivity & enables detection of low expressing genes
  • nCounter Gene Expression Assay Compatibility - Primer pools are available for all nCounter Gene Expression Panels; Custom designs are available

Product Information

  • Product Specs
  • Support Documents
  • Process Images
  • Ordering Information

Product Specifications

Feature Specifications
Input Amount
  • Purified Fresh/Frozen - 1ng
  • Cell Lysate - 1ng(~100 cells)
  • Purified FFPE - 10ng
# of Samples 48 reactions
Hands on Time 30 minutes
Assay Time 2.5 hours
Compatible Products
  • Pre-Configured NanoString Gene Expression Panels
  • Custom Panel Support
Storage Conditions
  • Low RNA Input Reagents - Store for up to 1 year at –20°C.
  • Panel Primer Pools - Stable for up to 1 year at -80°C.
  • Low RNA Input Kit Reagents and Panel-Specific Primer Pools are ordered individually shipped in separate boxes under different shipping conditions.

To receive a quote, please fill out the Request Information form.

Product Process images

Product Details

* : Panel-Specific Primer Pools for Use with Low RNA Input Kit + : Instrument Consumables for Use with Low RNA Input Kit
Product Name Description Quantity Catalog Number
CAE Panel Primer Pool MTE primer pool for Low Input RNA profiling 47 MAQC genes cancer-related human genes. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-CAE-12
*Human Cancer Reference Panel Primer Pool MTE primer pool for Low Input RNA profiling 233 cancer-related human genes. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-CR-12
*Human Stem Cell Panel Primer Pool MTE primer pool for Low Input RNA profiling 181 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-SC-12
*Human Immunology V2 Primer Pool MTE primer pool for Low Input RNA profiling (584 genes) 569 cancer-related human genes + 15 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-HIM2-12
*Human Inflammation V2 Primer Pool MTE primer pool for Low Input RNA profiling (249 genes) 243 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-HIN2-12
*Mouse Immunology V1 Primer Pool MTE primer pool for Low Input RNA profiling (559 genes) 549 cancer-related human genes + 14 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-MIM1-12
*Mouse Inflammation V2 Primer Pool MTE primer pool for Low Input RNA profiling (248 genes) 242 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-MIN2-12
*PanCancer Immune Profiling Panel Primer Pool MTE primer pool for Low Input RNA profiling (768 genes) 728 cancer-related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-HIP1-12
*PanCancer Pathways Panel Primer Pool MTE primer pool for Low Input RNA profiling (739 genes) 733 cancer-related human genes + 6 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-PATH-12
*PanCancer Progression Panel Primer Pool MTE primer pool for Low Input RNA profiling (770 genes) 730 cancer-related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-PROG1-12
*PanCancer Mouse Immune Profiling Panel Primer Pool MTE primer pool for Low Input RNA profiling (770 genes) 750 cancer-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-MIP1-12
*nCounter Human Myeloid Innate Immunity V2 Panel Primer Pool MTE primer pools for Low Input RNA profiling (770 genes) 730 immune related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-HMII2-12
*nCounter Mouse Myeloid Innate Immunity V2 Panel Primer Pool MTE primer pools for Low Input RNA profiling (754 genes) 734 immune-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required 12 reactions PP-MMII2-12
+nCounter Master Kit (Max or Flex Systems) Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems. 12 reactions NAA-AKIT-012
+nCounter SPRINT Cartridge Sample Cartridge for nCounter SPRINT System 12 reactions SPRINT-CAR-1.0
+nCounter SPRINT Reagent Pack nCounter SPRINT Reagent Pack containing Reagents A,B,C & Hybridization Buffer 192 reactions SPRINT-REAG-KIT

To receive a quote, please fill out the Request Information form.


Request Information | E-mail us: info@nanostring.com | Call us: 1 888 358 6266