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nCounter® Gene Expression CodeSets

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Unparalleled Performance in Digital Gene Expression

nCounter Gene Expression CodeSets offer a cost-effective way to analyze the expression levels of up to 800 genes simultaneously, with precision superior to qPCR. The system can directly assay tissue and blood lysates as well as Formalin-Fixed Paraffin-Embedded (FFPE) extracts in a simple workflow that requires only 15 minutes of total hands-on time per reaction.

Q&A with Dr. Lucas Dennis on Reproducibility

NanoString Technologies: Q&A with Dr. Lucas Dennis on Reproducibility

Product Highlights

  • Analyze up to 800 genes simultaneously
  • No RT, no enzymes, no amplification*
    *Single Cell analysis requires amplification prior to analysis on the nCounter System.
  • Direct input of multiple sample types including total RNA, cell lysate, FFPE samples,
    and whole-blood lysate
  • Single molecule digital counting minimizes data noise

Data You Can Count On

The nCounter Gene Expression Assay is designed to provide an ultra-sensitive, reproducible and highly multiplexed method for gene expression profiling across all levels of biological expression. This assay provides a method for direct detection of mRNAs with molecular barcodes called nCounter Reporter Probes without the use of reverse transcription or amplification.

Sensitivity, Reproducibility and Dynamic Range

Gene counts from two technical replicates plotted against one another demonstrate the assay reproducibility over a wide dynamic range (10 - 50,000 counts). One total RNA sample was split into two separate hybridization reactions and processed independently on the nCounter Analysis System. In this experiment, 75 counts is equal to a concentration of approximately 1 copy per cell. These data illustrate the high level of sensitivity and precision of the assay even at very low levels of expression.

Sensitivity, Reproducibility and Dynamic Range

  • Pre-built Panels

    Off-the-shelf CodeSets for a select set of gene panels that include all the consumables ready to perform gene expression analysis.

  • Custom CodeSets

    Custom CodeSets provide the ultimate in flexibility and can be designed for specific genes, organisms, and hard to target sequences




nCounter® Gene Expression CodeSets Specifications

  • Specifications
  • Ordering Info

Specifications

Description Specifications
Level of multiplexing 20 - 800 gene targets
Recommended amount of starting material 100ng purified total RNA, or lysate from ~10,000 cells
Sample types supported Total RNA, cell lysates in GITC, FFPE-derived total RNA and PAXgene lysed whole blood, amplified RNA
Reaction volume 30 μL
Limit of detection 0.5fM spike-in control (~1 copy per cell); 90% of the time
Fold-change sensitivity > 1.5 fold (> 5 copies per cell)
> 2 fold change (> 1 copy per cell)
Spike correlation R² ≥ 0.95
Linear dynamic range 7 x 10⁵ total counts
Controls 6 positive controls
8 negative controls

To receive a quote, please fill out the Request Information form.

Ordering Information

Description Quantity / Use Part Number (P/N)
nCounter Gene Expression Custom CodeSet 48 assays
96 assays
192 assays
384 assays
576 assays
GXA-P1CS-048
GXA-P1CS-096
GXA-P1CS-192
GXA-P1CS-384
GXA-P1CS-576
nCounter CAE Kit
(expression profiling of 48 human MAQC genes)
12 assays
24 assays
48 assays
96 assays
GXA-CAE-12
GXA-CAE-24
GXA-CAE-48
GXA-CAE-96
nCounter GX Human Kinase Kit
(expression profiling of 519 human kinase genes)
12 assays
24 assays
48 assays
96 assays
GXA-P2K1-12
GXA-P2K1-24
GXA-P2K1-48
GXA-P2K1-96
nCounter Human Reference GX Kit
(expression profiling of 18 endogenous human reference genes)
12 assays
24 assays
48 assays
96 assays
GXA-P1HR-12
GXA-P1HR-24
GXA-P1HR-48
GXA-P1HR-96
nCounter GX Human Immunology Kit
(gene expression CodeSet profiling over 500 immunology genes; includes all consumables and reagents)
12 assays
24 assays
48 assays
96 assays
GXA-HIM1-12
GXA-HIM1-24
GXA-HIM1-48
GXA-HIM1-96
nCounter Human Cancer Reference Kit
(gene expression CodeSet profiling 230 cancer-related human genes; includes all consumables and reagents)
12 assays
24 assays
48 assays
96 assays
GXA-CR1-12
GXA-CR1-24
GXA-CR1-48
GXA-CR1-96
nCounter Human Inflammation Kit
(gene expression CodeSet profiling 184 inflammation-related human genes; includes all consumables and reagents)
12 assays
24 assays
48 assays
96 assays
GXA-IN1-12
GXA-IN1-24
GXA-IN1-48
GXA-IN1-96
nCounter Mouse Inflammation Kit
(gene expression CodeSet profiling 179 inflammation-related mouse genes; includes all consumables and reagents)
12 assays
24 assays
48 assays
96 assays
GXA-MI1-12
GXA-MI1-24
GXA-MI1-48
GXA-MI1-96
nCounter Analysis System
(includes the Prep Station and Digital Analyzer)
1 NCT-SYS-120
Additional nCounter Prep Station 1 NCT-PREP-120
Additional nCounter Digital Analyzer 1 NCT-DIGA-120

To receive a quote, please fill out the Request Information form.


Request Information | E-mail us: info@nanostring.com | Call us: 1 888 358 6266