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"nCounter microRNA panels have allowed us to comprehensively screen human and rat tissue for microRNA profiles in our disease setting of interest. This has allowed us to select candidate microRNA signatures of interest for expanded validation and further interrogation."
- Rachel Crossland, Ph.D., Newcastle University
nCounter miRNA Expression Assays provide highly multiplexed, direct digital detection and quantification of miRNAs in a single reaction without amplification. They allow researchers to produce highly accurate, reliable, and actionable biological information from low amount of samples in an integrated workflow compared to other technologies such as qPCR, NGS, and microarray.
nCounter miRNA Expression Assays include up to 800 biologically actionable genes curated from the latest miRbase (V21). Assays were selected based on published evidence of biological relevance and performance metrics.
NanoString’s FFPE compatible chemistry allows for tens of thousands of data-points to be generated per day in clinically-relevant sample types important in translational research.
The discovery of relatively stable, extracellular miRNAs in serum and plasma has generated huge interest as non-invasive biomarkers for a variety of diseases.
Exosomal miRNAs play an important role in disease progression, and can stimulate angiogenesis and facilitate metastasis in cancers
miRNAs have been identified as potential cancer biomarkers due to their presence and stability in many body fluids including urine.
Christmann R. et al. (2016) performed biomarker analysis on tissue samples from patients with SSc-ILD to find miR-155 in the progression of lung fibrosis in systemic sclerosis using miRNA Expression Human v3 Assays.
Luciano C. et al. (2013) found interesting integrated miRNA and mRNA signatures associated with survival in triple-negative breast cancer using the miRGE™ Assays (simultaneous analysis of miRNA and mRNA on the same sample).
Topol A. et al. (2016) used the nCounter miRNA Expression Human v3 Assay to study dysregulation of miRNA-9 in a subset of schizophrenia patent-derived neural progenitor cells
Rodosthenis S. et al. (2016) performed a pilot study of older individuals by studying ambient particulate matter and miRNA in extracellular vesicles.
Permuth-Wey J. et al. (2015) discovered novel miRNA biomarkers in plasma samples for patients with intraductal papillary mucinous neoplasms of the pancreas using nCounter miRNA Expression Human Assays v3.
Stylii SS et al. (2016) profiled miRNA in cerebrospinal fluid in patients with aneurysmal subarachnoidhemorrhage using nCounter miRNA Expression Human v3 Assays.
NanoString profiling indicates differential serum expression levels of circulating miRNAs in healthycontrols and children with mild or severe symptoms of EV71. Read more in Elevated expression of circulating miR876-5p is a specific response to severe EV71 infections by Wang R. et. al. (2016).
Armstrong D.A. et al. (2015) profiled miRNA from matched tumor and bio-fluids in bladder cancer using nCounter miRNA Expression Human v3 Assays.
See the complete list of publications using nCounter miRNA Expression Assays here.
Highly sensitive and specific quantification of miRNA in serum and plasma samples.
Automated past-hybridization processing on nCounter systems.
nSolver utilizes a variety of appropriate normalization methodologies in all samples to provide accurate analysis.
The nCounter miRGE™ Assay is uniquely designed to provide a single-tube, sensitive, reproducible and highly multiplexed method for detecting subsets of both mRNAs and miRNAs simultaneously across all biological levels of expression. To learn more, please visit the nCounter miRGE CodeSets product page.
Webinar Live during: Jun 21, 2016 at 6:00am
6am PST | 9am PST (Seattle)
2pm BST | 3pm CEST 5pm BST (England, Scotland)
By Kirsteen Maclean , Sr. Field Application Scientist
*Registration required to watch on demand.
Off-the-shelf kits arrive at your lab with the highly multiplexed CodeSet and all of the reagents and consumables necessary to perform the assay. Kits are available for 12 reactions and multiple other scales, offering flexibility for labs and projects of all sizes.
To receive a quote, please fill out the Request Information form.
nCounter Human miRNA Assay Kits
nCounter Human miRNA Assay Kits Includes 800 human miRNAs
(based on miRBase v21)
(based on miRBase v21)
nCounter Mouse miRNA Assay Kits
Includes more than 600 mouse and mouse-associated viral miRNAs derived from miRBase.
nCounter Rat miRNA Assay Kits
Includes more than 400 Rat miRNAs derived from miRBase.
nCounter Custom à la carte miRNA CodeSets
nCounter Custom à la carte miRNA CodeSets available for analysis of subsets of miRNAs included in species-specific miRNA Expression Assay Kits. Contact firstname.lastname@example.org for more information.
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