Turn discoveries into practical tools for routine analysis
Bock C, et al., “Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines” Cell 144, 439-452. February 4, 2011
In this recent Cell paper, Bock et al., assert that before embryonic stem (ES) or induced pluripotent stem (iPS) cells can be confidently used in translational research, differences of gene expression methylation patterns among different cell types and at different stages of differentiation must be better understood. To this end, they analyzed 500 genes across 20 previously derived human ES lines and 12 human iPS cell lines via nCounter, sequencing, and arrays. Using this data, they performed analysis to develop “a scorecard for quick and comprehensive characterization of pluripotent stem cells”.
“To be practically useful”, the authors state, “such a lineage scorecard cannot rely on expensive and time-consuming directed differentiation protocols. Instead we chose a simple nondirected EB differentiation assay and combined it with highly quantitative gene expression profiling (nCounter) and bioinformatics algorithm.”
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