You are here:
Sectional Navigation
The Benefits of Digital Add Up...
- Multiplex hundreds of gene targets in a single reaction
- High Sensitivity (<1 copy per cell)
- Fully-automated system
- Exceptionally easy-to-use
- No enzymes required to perform assay
Innovation after Innovation after Innovation...and Counting
The nCounter Analysis System utilizes a novel digital technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (<1 copy per cell). The technology uses molecular "barcodes" and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.
Each color-coded barcode is attached to a single target-specific probe corresponding to a gene of interest. Mixed together with controls, they form a multiplexed CodeSet.
Hybridization
NanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection.
Purification and Immobilization
After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge.
Data Collection
Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule.
nCounter Analysis System
The nCounter Analysis System does not rely on enzymes for processing or amplification. It enables highly sensitive detection and quantification of gene expression from a wide variety of sample types including direct measurement from purified total RNA, cell and tissue lysates, RNA extracted from FFPE samples and blood without globin mitigation.
The nCounter Analysis System is an integrated system comprised of a fully-automated Prep Station, a Digital Analyzer, the CodeSet (molecular barcodes) and all of the reagents and consumables needed to perform the analysis.
Join our mailing list
Get news from us on software releases, new innovations and upcoming events!