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Immuno-Oncology Profiling

REQUEST INFO RNA: PROTEIN WHITE PAPER IMMMUNO-ONCOLOGY WHITE PAPER PBMC TIPS WHITE PAPER

Don’t let sample volume limit your analytical aspirations. Quantify DNA, RNA, and protein in a single assay to use less precious material and gather more informative data.

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SIMULTANEOUS ANALYSIS OF GENE AND PROTEIN EXPRESSION

The nCounter® Vantage 3D™ RNA:Protein Immune Cell Profiling Assay combines our PanCancer Immune Profiling panel for RNA expression analysis with 30 key immuno-oncology protein targets and allows for direct measurement of RNA and protein expression using the proven nCounter system. Enabling a new way of looking at your targets and systems of interest, you can get more data with less sample and gain deeper insight into your immuno-oncology research.

Stage of Cancer
Immunity Cycle
Proteins in the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay
2 Antigen Presentation CD4, CD40, CD154 (CD40L)
3 Priming and Activation CD279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD25 (IL2R), CD56 (NCAM), CD357 (GITR), CD137 (OX40), CD27, CD28, CD127, CD137 (4-1BB)
4-5 Trafficking and Infiltration CD9
6-7 Recognition and Killing of
Cancer Cells
CD279 (PD-1), CD274 (PD-L1), CD273 (PD-L2), CD272 (BTLA), HLA-DRA
Immune Modulation CD279 (PD-1), CD278 (ICOS), CD158e1 (KIR3DL1), CD335 (NKp46), CD152 (CTLA-4), CD3 (CD3E), CD8 (CD8A), CD14, CD19, CD33, CD68, CD163

BENCHMARKING TO GOLD STANDARD TECHNIQUES

Combined RNA and protein profiling reveals potential post-transcriptional regulation for a subset of targets and protein expression data that is highly correlated with gold standard flow cytometry.

PBMCs were stimulated with PMA and RNA and protein expression was profiled using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay (input of 50,000 PBMCs). Additionally, a subset of protein targets were analyzed by flow cytometry and data from both platforms showed high correlation.

CHARACTERIZING MACROPHAGE DIFFERENTIATION AND POLARIZATION

Integrated analysis enables simultaneous quantitation of RNA and protein expression from a single sample and eliminates the need to compare data from different technologies with different outputs.

Time-course data from 0-96 hours, giving a deeper view of the changes in gene and protein co-expression.
Time-course data from 0-96 hours, giving a deeper view of the changes in gene and protein co-expression.


A THP-1 monocytic cell line was induced to differentiate into a macrophage phenotype with PMA. Cells were then polarized to an M1 or M2 phenotype by 24h culture using IFNγ + LPS, or IL4, respectively. Gene and protein expression was characterized at each stage of differentiation using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay.

PRECISE CELL PROFILING

RNA:Protein signatures can accurately identify specific immune cell types, allowing you to uncover changes in a heterogeneous cell population in response to external stimuli, such as immunotherapeutic agents, for example.

To learn more about our nCounter Vantage 3D Portfolio, click here.


FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.


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